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miRanalyser
miRBase
Rfam
UCSC
miRDeep
Small RNA annotation using one sample
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(recommend)
Sample upload and reference genome selection:
Reference genome:
Arabidopsis thaliana(Build 9.1)
Caenorhabditis elegans(WS190)
Chicken(galGal3)
Chimpanzee(panTro2)
Cow(bosTau4)
Dog(canFam2)
Horse(equCab2)
Human(hg18)
Mouse(mm9)
Opossum(monDom5)
Orangutan(ponAbe2)
Oryza sativa(RAP Build 3)
Rat(rn4)
Rhesus(rheMac2)
Zebrafish(Zv8)
Length of interval:
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
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16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
Sample data:
(*.fa or *.gz or *.zip, Max 10M )
Hide Parameters
Show Parameters
Parameters for SOAP to align reference genome:
M value:
0
1
2
4
Match mode: 0=exact; 1=1 mismatch; 2=2 mismatch; 4=the best
R value:
0
1
2
How to report repeat hits: 0=none; 1=random one; 2=all
V value:
0
1
2
3
4
Maximum number of mismatches
Parameters for MEGABLAST to align miRBase and Rfam:
E value:
1
0.1
0.01
0.001
0.0001
Expect value
B value:
1
2
3
4
5
Number of database sequence to show alignments for (B)
V value:
1
2
3
4
5
Number of database sequences to show one-line descriptions for (V)
Parameters for miRDeep to predict novel miRNA:
L value:
Flank sequence length of the query. Range 0 - 200
test
Institute of Genomic Medicine/Zhejiang Provincial Key Laboratory of Medical Genetics,
Wenzhou Medical College, Wenzhou 325035, China
