Main Menu
Home
Filter
Single
Multiple
Group
Retrieve
Help
Feedback
About Us
Useful Links
miRanalyser
miRBase
Rfam
UCSC
miRDeep
Small RNA annotation using two samples
Enter your email address
(recommend)
Sample upload and reference genome selection:
Reference genome:
Arabidopsis thaliana(Build 9.1)
Caenorhabditis elegans(WS190)
Chicken(galGal3)
Chimpanzee(panTro2)
Cow(bosTau4)
Dog(canFam2)
Horse(equCab2)
Human(hg18)
Mouse(mm9)
Opossum(monDom5)
Orangutan(ponAbe2)
Oryza sativa(RAP Build 3)
Rat(rn4)
Rhesus(rheMac2)
Zebrafish(Zv8)
Length of interval:
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
--
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
Sample A data: (control)
Sample B data:
(*.fa or *.gz or *.zip, Max 20M in total)
Hide Parameters
Show Parameters
Parameters for SOAP to align reference genome:
M value:
0
1
2
4
Match mode: 0=exact; 1=1 mismatch; 2=2 mismatch; 4=the best
R value:
0
1
2
How to report repeat hits: 0=none; 1=random one; 2=all
V value:
0
1
2
3
4
Maximum number of mismatches
Parameters for MEGABLAST to align miRBase and Rfam:
E value:
1
0.1
0.01
0.001
0.0001
Expect value
B value:
1
2
3
4
5
Number of database sequence to show alignments for (B)
V value:
1
2
3
4
5
Number of database sequences to show one-line descriptions for (V)
Parameters for miRDeep to predict novel miRNA:
L value:
Flank sequence length of the query. Range 0 - 200
Parameters for detection differentially expressed miRNAs:
P value:
0.05
0.01
0.005
0.001
P value inferred based on Bayesian method
D value:
1
1.5
2
2.5
3
3.5
4
Fold change in normalized sequence counts
test
Institute of Genomic Medicine/Zhejiang Provincial Key Laboratory of Medical Genetics,
Wenzhou Medical College, Wenzhou 325035, China
