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  Small RNA annotation using two samples

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Sample upload and reference genome selection:    
   
Reference genome:
 
Length of interval: --
 
Sample A data: (control)

     

Sample B data:

(*.fa or *.gz or *.zip, Max 20M in total)
 
    Hide Parameters
Parameters for SOAP to align reference genome:
     
M value: Match mode: 0=exact; 1=1 mismatch; 2=2 mismatch; 4=the best
R value: How to report repeat hits: 0=none; 1=random one; 2=all
V value: Maximum number of mismatches
Parameters for MEGABLAST to align miRBase and Rfam:
     
E value: Expect value
B value: Number of database sequence to show alignments for (B)
V value: Number of database sequences to show one-line descriptions for (V)
Parameters for miRDeep to predict novel miRNA:
     
L value: Flank sequence length of the query. Range 0 - 200
Parameters for detection differentially expressed miRNAs:
     
P value: P value inferred based on Bayesian method
     
D value: Fold change in normalized sequence counts
 
 
Institute of Genomic Medicine/Zhejiang Provincial Key Laboratory of Medical Genetics,
Wenzhou Medical College, Wenzhou 325035, China